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Article

English

ID: <

10.12681/jhvms.17542

>

·

DOI: <

10.12681/jhvms.17542

>

Where these data come from
Molecular analysis of Peste des Petits Ruminants Virus from outbreak in Turkey during 2010-2012

Abstract

The first aim is to detect PPRV in specimens obtained from small ruminants during 2010-12 using RT-PCRs. The second aim is to identify the epidemiological relationships of the detected PPRV strains among them, as well as between other strains.In this study blood, swab and tissue samples (n=574) taken from sheep (n=473) and goats (n=101) suspected of having PPRV infection from an outbreak in 50 different provinces of Turkey during 2010-12 were tested based on the F and N gene regions by reverse transcription PCR (RT-PCR) and real-time reverse transcription PCR (RT-qPCR). Positivity ratios were 35.5% with F gene RT-PCR, 39.3% with N gene RT-PCR and 44.4% with N gene RT-qPCR, giving an overall positivity rate of 45.8%.Of the positive samples, 53 for F-gene and 60 for N-gene representing different provinces were selected. After phylogenetic analysis, the circulating PPRV was located in lineage IV according to two gene regions. The F-gene partial sequence analysis at the nucleotide level showed 98.2-100% nucleotide sequence identity among 53 for F-gene, and 97.9-98.9% and 91.3-92.4% to Turkey2000 and Nigeria75/1 sequences, respectively. The N-gene partial sequence analysis at the nucleotide level showed 94.2-100% resemblence among 60 for N-gene, and 94.2-98.3% and 89.3-90.9% to Turkey2000 and Nigeria75/1 sequences, respectively.These results indicated that PPRV infection was endemic in Turkey. The causative agent belonged to the Lineage 4 which had three different haplogroup and the illegal animal movement was an importand vehicle of the virus from different sources according to the philogenetic analyses.

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