This work develops an analysis of the concept of social participation in numerous research under the EcoHealth approach on diseases such as malaria, dengue and Chagas’ disease in Latin America. In addition, the strategies implemented, material and methods used are discussed, highlighting qualitative exploratory studies, through narrative analysis, using guides that seek to identify aspects related to social participation; research results highlight the different levels of development of the concept of participation among the different population groups that were the subject of the study. Finally, the findings of the research outlined above have as their common denominator the establishment of social participation as a key element for the sustainability of the projects implemented, as well as the principles of the ecosystem approach.