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Article

Portuguese

ID: <

10670/1.5h57ok

>

·

DOI: <

10.34117/bjdv6n7-794

>

Where these data come from
Detection and partial molecular characterisation of a Badnavirus isolate in Brazil/Detection and partial molecular characterisation of an isolate from Badnavvirus in Brazil

Abstract

sweet potatoes are one of the most important plants in the world and are one of the main sources of nutrients in some countries. Although it is considered to be a resistant plant, it hosts several pathogens, mainly viruses. Previously, most viruses reported in the harvest had a genome composed of RNA, but DNA viruses have emerged in recent years. These include species belonging to the gênero Badnavirus (family Caulimoviridae). Therefore, the purpose of this study was to identify the presence of viral species belonging to the gênero Badnavirus in samples of sweet leaves collected in the main producing regions of the city of Pelotas — RS. Through PCR amplification, 48 positive samples out of the 303 tested samples could be detected using degenerated and species-specific primers. One sample has been selected and used for analysis with other specific primers pairs to obtain genome sequences. However, it was not possible to obtain the complete sequence of the genome, only partial. The fragments generated showed an identity of between 72 and 87 % when compared to the full genome of SPV-A (FJ560943.2) and SPV-B (FJ560944.1) isolates, demonstrating the great variability between them. In addition, studies will be carried out to obtain the complete genome sequence to better understand the action of the virus on the plant.

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