Balanced nutrition is the key point of a healthy life includes intake of vitamins and minerals. Vitamins such as folate (B9) have an important role in system homeostasis. Vitamin B derivatives, also folate are water-soluble vitamin class which plays a key role in cell metabolism. Folate is necessary to produce new cells via stimulating DNA and RNA methylation. Folate has positive effect on recurrent aphthous stomatitis, gingival hyperplasia, preventing early childhood caries and periodontal diseases. Alveolar bone and periodontal ligament development are related to sufficient concentrations of folate. Folate reduces gum bleeding, and increases osteoblastic activity and bone mineral density, also decreases osteoclastic activity. Effect on DNA and RNA metabolism causes the reduction of reactive oxygen species. In early stages of pregnancy, folate deficiency may cause birth anomalies due to neural tube defects such as lip, alveolar and palatal clefts. Folate deficiency effects on DNA and RNA metabolism negatively. DNA and RNA repair, production and methylation system is being interrupted. Therefore chromosal abnormalities occur and that situation may cause cancer and leukemia. Folate is mainly provides systemic homeostasis and important for maintaining chromosomal activities. Consequently adequate concentrations of folate must be taken regularly.