[EN] The analysis of organic contaminants in environmental samples usually requires the use of separation techniques, given the complexity of the samples and the large number of compounds of interest present in them. High performance liquid chromatography (HPLC) is the preferred technique, but the development of suitable stationary phases remains a challenge for Analytical Chemistry. In this work, the feasibility of using ethylene tetrafluoroethylene (ETFE) tubes as a support in the preparation of monolithic columns by photopolymerization has been studied. The optimization of the polymerization mixture, based on methacrylate esters, has allowed the development of a monolithic column that has provide a good separation of organic compounds such as alkylbenzenes, polycyclic aromatic hydrocarbons (PAHs) and proteins. The proposed synthesis process has presented a high reproducibility and allows to obtain, in a fast way (thanks to the use of ultraviolet light to start the polymerization process) and easily ( accessible in any laboratory), monolithic columns for use in the conventional HPLC. Furthermore, a first attempt has been made to obtain a monolith capable of separating organic compounds with high polarity. TFGM