[EN] Wild tomato relatives are commonly used in breeding programs to widen the genetic basis of the cultivated species (Solanum lycopersicum) and make use of interesting genes. Among all related species, S. peruvianum has several characteristics of interest related with resistance to biotic and abiotic stresses. In particular, the accession PI 126944 has been described as resistant to several diseases. This species is one of the most distantly related to the cultivated tomato within the Lycopersicon group of the Lycopersicon section where both belong. This accession presents crossability barriers with the cultivated species, and special techniques, such as embryo rescue and in vitro culture are necessary to obtain interespecific hybrids. In previous research developed in the ¿Instituto Universitario de Conservación y Mejora de la Agrodiversidad¿ (COMAV), three interespecific hybrids from this accession were obtained and several generations derived from them were available at the beginning of this work. The aim of this work was the initial development of the ILs derived from the S. peruvianum accession PI 126944. Specifically, the objective were to genotype the most advanced generations derived from PI 126944 with available molecular markers, to backcross to cultivated tomato available generations using embryo rescue technique as well as to begin the genotyping of the obtained plants by this technique. Genetic material used in the present work was based on several generations derived from the interspecific hybrid between tomato and PI 126944: 18 plants of the generation BC2, four plants of F3-BC1, nine plants of F6,seven plants of the generation F5 and 20 plants of F2-BC1. It was necessary to determine the parents from which some of the generations came..Two generations (BC2 and F6) were genotyped with SSR and CAPS molecular, previously described as polymorphic between tomato and PI 126944. Considering all analyzed generations, alleles from the wild species were found. With embryo rescue, fruits were obtained from backcross developed with F2, F5, F3-BC1 x F3-BC1, BC2, F6 and F4. However, it was only possible to regenerate plants from crosses between FC and F5 and BC2 generations. In future works, these plants will be genotyped.