[EN] A collection of introgression lines (ILs) of Solanum lycopersicoides Dunal in the genetic background of cultivated tomato (S. lycopersicum L.) is a valuable tool for tomato breeding. Efficient management of the collection requires the use of molecular markers. The objective of this work was to identify polymerase chain reaction (PCR)-based markers that were polymorphic between the parents of the ILs, namely ‘VF36’ and ‘LA2951’. In total, 81 primer pairs were tested on genomic DNA from both parents. Genomic DNA of the inter-specific hybrid between the two parents, from which the IL collection had been derived, was also tested. The markers used were either cleaved amplified polymorphic sequence (CAPS) markers or were derived from a set of conserved orthologous genes. In both cases, the markers had been mapped in tomato and described in the SOL Genomics Network. Forty-seven of the markers tested produced a single PCR product in ‘VF36’ and ‘LA2951’. Eleven markers revealed polymorphisms as differences in band-sizes between the two parents. At least one restriction enzyme that generated polymorphism was identified in 29 of the remaining 36 markers. Among other applications, some of these markers have been used to identify plants carrying a target DNA fragment among segregating generations, or to delimit the length of an introgressed sequence This research was financed by the Ministerio de Educacion y Ciencia and Ministerio de Ciencia e Innovacion, Madrid, Spain (Projects Nos. AGL2008-05114 and AGL2011-30083). Peiró Barber, RM.; Díez Niclós, MJTDJ.; Pérez De Castro, AM. (2015). A set of PCR-based markers for management of a library of Solanum lycopersicoides introgression lines. The Journal of Horticultural Science and Biotechnology. 90(3):279-284. https://doi.org/10.1080/14620316.2015.11513183 Díez, M. J. and Nuez, F. (2008). Tomato. In:Handbook of Plant Breeding. (Prohens, J. and Nuez F., Eds.). Springer, New York, NY, USA, 249–323. Pérez-De-Castro, A., Díez, M. J. and Nuez, F. (2011). Evaluation of a subset ofSolanum lycopersicoidesintrogression lines for resistance to Tomato yellow leaf curl disease. In:Proceedings of the XVII Eucarpia Meeting Group - Tomato. Málaga, Spain. 17.

Soler, S., Belmonte, I., Aramburu, J., Galipienso, L., López, C., Sifres, A., Pérez-De-Castro, A. and Díez, M. J. (2012). Identificación de una fuente de tolerancia al ToMV en una colección de líneas de introgresión derivada deSolanum lycopersicoidesLA2951. In:Proceedings of the XVI Congreso de la Sociedad Española de Fitopatología, Málaga, Spain. 221.

Soler, S., Belmonte, I., Aramburu, J., Galipiensos, L., López, C., Sifres, A., Pérez-De-Castro, A. and Díez, M. J. (2012). Identification of a ToMV tolerance source in a collection of derived intrusion lines from Solanum lycopersicoidesLA2951. In: Proceedings of the XVI Congress of the Spanish Phytopathology Society, Malaga, Spain. 221.

A set of PCR-based markers for management of a library of Solanum lycopersicoides introgression lines

Abstract