Fast urbanization has led to ozone (O3) being the main pollutant in summer in most of China. To assess future ground-level O3 effects on the service of urban greening species and clarify the underlying mechanism of O3 damage, four common urban greening species, Ailanthus altissima (AA), Fraxinus chinensis(FC), Platanus orientalis (PO) and Robinia pseudoacacia (RP) were exposed to non-filtered air (NF) and to elevated O3 (E-O3) in open-top chambers. E-O3 induced visible injury in all species as well as microscopic alterations such as collapse of the palisade parenchyma cells, callose accumulation, or chloroplast and mitochondrial accelerated senescence. E-O3 significantly reduced light-saturated CO2 assimilation (Asat),the maximum activity of Rubisco (Vcmax), the maximum electron transport rate (Jmax), and fluorescence parameters such as the quantum yield of noncyclic electron transport (�PSII), and the quenching of photochemical efficiency of PSII (qP). It also increased total antioxidant capacity, phenolics and ascorbate contents. No significant interaction between O3and species was found in photosynthetic performance and antioxidant systems, suggesting that the four species selected were sensitive to O3. Of all four species,AA was the most sensitive species due to a combination of earlier injury onset, anatomical features, lower antioxidant responses and higher stomatal conductance. The sensitivity of tree species to O3 is a factor to be considered for urban greening. Ozone may affect important urban forest ecosystem services by reducing CO2 assimilation This study has been funded by the Hundred Talents Program, Chinese Academy of Sciences and State Key Laboratory of Urban and Regional Ecology. Collaboration between RCEES and Fundacion CEAM has been possible thanks to project AMIS (Fate and Impact of Atmospheric Pollutants, PIRSES-GA-2011-295132), and by the Chinese Academy of Sciences Visiting Professorships for Senior International Scientists (grant number: 2013T2Z0009). VC also acknowledges the support of PROMETEOII/2014/038 project (Generalitat Valenciana, G.V.), and FGB and JRA that of PROMETEOII2013/021 (G.V.), and CGL2012-40058-C02-01/02 (MINECO). We thank Mr. Yulong Zhang for the experimental management. Gao, F.; Calatayud Lorente, V.; García-Breijo, F.; Reig Armiñana, J.; Feng, Z. (2016). Effects of elevated ozone on physiological, anatomical and ultrastructural characteristics of four common urban tree species in China. Ecological Indicators. 67:367-379. doi:10.1016/j.ecolind.2016.03.012