Search publications, data, projects and authors
Pattern of genetic variability of Solanum habrochaites in its natural area of distribution


KeywordsTriple Keywords
Populations, Human
Population growth
Human populations
Human population
Species distribution
Geographical distribution of animals and plants
Species--Geographical distribution
Areography (Biology)
Appearance (Philosophy)


The tomato wild relative species Solanum habrochaites (previously known as Lycopersicon hirsutum) is a potential source of novel genes for tomato breeding. It shows resistance to many diseases and pests, cold tolerance and fruit quality traits. This species inhabits the western Andean slopes at high elevations from central Ecuador to central Peru. In this study the genetic variation of S. habrochaites was studied using 91 accessions from the whole range of distribution of this species. To this end, we employed two kinds of markers: 9 SSRs and 6 AFLP combinations. The Principal Coordinate Analysis based on AFLP data showed the existence of clinal genetic variation from north to south. The accessions of the different geographic groups were sequentially arranged in the first axis from north to south and a clear separation between them was found. The groups from the centre of the area of distribution showed the highest variation and heterozygosis. The ones from the margins showed lower variability and presented higher homozygosis. The morphotypes typicum and glabratum considered by Müller constituted the extreme forms of the continuous variation in the pubescence grade. These differences in pubescence were not associated with the homozygosis grade. © 2010 Springer Science+Business Media B.V. Sifres Cuerda, AG.; Blanca Postigo, JM.; Nuez Viñals, F. (2011). Pattern of genetic variability of Solanum habrochaites in its natural area of distribution. Genetic Resources and Crop Evolution. 58(3):347-360. doi:10.1007/s10722-010-9578-0 Baker HG (1955) Self-compatibility and establishment after long-distance dispersal. Evolution 9:347–348 Benham J, Jeung JU, Jasieniuk M, Kanazin V, Blake T (1999) Genographer: a graphical tool for automated fluorescent AFLP and microsatellite analysis. Department of Plant Science, Montana State University, Bozeman Bernacchi D, Beck-Bunn T, Eshed Y, Lopez J, Petiard V, Uhlig J, Zamir D, Tanksley S (1998) Advanced backcross QTL analysis in tomato. I. Identification of QTLs for traits of agronomic importance from Lycopersicon hirsutum. Theor Appl Genet 97:381–397 Bornet B, Goraguer F, Joly G, Brachard M (2002) Genetic diversity in European and Argentinian cultivated potatoes (Solanum tuberosum subsp. tuberosum) detected by inter-simple sequence repeats (ISSRs). Genome 45:481–484 Bouxin G (2005) Ginkgo, a multivariate analysis package. J Veg Sci 16:355–359 Cavalli-Sforza LL, Edwards AWF (1967) Phylogenetic analysis: models and estimation procedures. Evolution 32:550–570 Coulibaly S, Pasquet R, Papa R, Gepts P (2002) AFLP analysis of the phenetic organization and genetic diversity of Vigna unguiculata L. Walp. reveals extensive gene flow between wild and domesticated types. Theor Appl Genet 104:358–366 Ercolano MR, Sebastiano A, Monti L, Frusciante L, Barone A (2005) Molecular characterization of Solanum habrochaites accessions. J Genet Breed 59:15–20 Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): an integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50 Felsenstein J (1993) PHYLIP (Phylogeny Inference Package) v3.69. Department of Genome Sciences and Department of Biology. University of Washington. Washington. EEUU. Website http://www.evolution.genetics.washington.edu/phylip.html Ferriol M, Pico B, Nuez F (2003) Genetic diversity of a germplasm collection of Cucurbita pepo using SRAP and AFLP markers. Theor Appl Genet 107:271–282 Foolad MR, Merk HL, Ashrafi H (2008) Genetics, genomics and breeding of late blight and early blight resistance in tomato. Crit Rev Plant Sci 27:75–107 Gower JC (1966) Some distance properties of latent roots and vector methods used in multivariate analysis. Biometrika 53:325–338 Guo ZH, Weston PA, Snyder JC (1993) Repellency to 2-spotted spider-mite, Tetranychus urticae Koch, as related to leaf surface-chemistry of Lycopersicon hirsutum accessions. J Chem Ecol 19:2965–2979 Kabelka E, Franchino B, Francis DM (2002) Two loci from Lycopersicon hirsutum LA407 confer resistance to strains of Clavibacter michiganensis subsp. michiganensis. Phytopathology 92:504–510 Langella O (2002) Populations 1.2.28, Population genetic software. CNRS Website http://www.bioinformatics.org/~tryphon/populations/ Leite G, Picanco M, Guedes R, Zanuncio J (2001) Role of plant age in the resistance of Lycopersicon hirsutum f. glabratum to the tomato leafminer Tuta absoluta (Lepidoptera: Gelechiidae). Sci Hortic 89:103–113 Mantel N (1967) The detection of disease clustering and a generalized regression approach. Cancer Res 27:209–220 Momotaz A, Scott JW, Schuster DJ (2005) Searching for silverleaf whitefly and begomovirus resistance genes from Lycopersicon hirsutum accession LA1777. Acta Hort 695:417–422 Monforte A, Tanksley S (2000) Development of a set of near isogenic and backcross recombinant inbred lines containing most of the Lycopersicon hirsutum genome in a L. esculentum genetic background: a tool for gene mapping and gene discovery. Genome 43:803–813 Müller CH (1940) A revision of the genus Lycopersicon. United States Department of Agriculture. Misc Publ No 382 Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70:3321–3323 Nuez F, Prohens J, Blanca JM (2004) Relationships origin, and diversity of Galapagos tomatoes: implications for the conservation of natural populations. Am J Bot 91:86–99 Nuez F, Diez MJ, Valcarcel JV, Cebolla-Cornejo J, Perez A, Soler S, Rosello S, Adalid A, Galiana L, Sifres A, Pico B, Blanca JM, Frutos R (2008) Genetic resources of Lycopersicon at the institute for the conservation and improvement of the agrodiversity. Acta Hortic 789:293–297 Peralta IE, Spooner DM, Knapp S (2008) The taxonomy of tomatoes: a revision of wild tomatoes (Solanum section Lycopersicon) and their outgroup relatives in sections Juglandifolium and Lycopersicoides. Syst Bot Monogr 84:1–186 + 3 plates Phillips N, Larson S, Drost D (2008) Detection of genetic variation in wild populations of three Allium species using amplified fragment length polymorphisms. HortScience 43:637–643 Pritchard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959. http://www.pritch.bsd.uchicago.edu/structure.html Qian H, Ricklefs RE, White PS (2005) Beta diversity of angiosperms in temperate floras of eastern Asia and eastern North America. Ecol Lett 8:15–22 Rick CM (1982) The potential of exotic germplasm for tomato improvement. In: Vasil IK, Scowcroft WR, Frey KJ (eds) Plant improvement and somatic cell genetics. Academic Press Inc., New York, pp 1–28 Rick CM, Fobes JF, Holle M (1977) Genetic variation in Lycopersicon pimpinellifolium: evidence of evolutionary change in mating systems. Plant Syst Evol 127:139–170 Rick CM, Fobes JF, Tanksley SD (1979) Evolution of mating systems in Lycopersicon hirsutum as deduced from genetic variation in electrophoretic and morphological characters. Plant Syst Evol 132:279–298 Sacks E, St. Clair D (1998) Variation among seven genotypes of Lycopersicon esculentum and 36 accessions of L. hirsutum for interspecific crossability. Euphytica 101:185–191 Sifres A, Pico B, Blanca J, De Frutos R, Nuez F (2007) Genetic structure of Lycopersicon pimpinellifolium (Solanaceae) populations collected after the ENSO event of 1997–1998. Genet Resour Crop Evol 54:359–377 Smulders MJM, Bredemeijer G, Rus-Kortekaas W, Arens P, Vosman B (1997) Use of short microsatellites from database sequences to generate polymorphisms among Lycopersicon esculentum cultivars and accessions of other Lycopersicon species. Theor Appl Genet 97:264–272 Snyder JC, Guo ZH, Thacker R, Goodman J, Stpyrek J (1993) 2, 3-Dihydrofarnesoic acid, a unique terpene from trichomes of Lycopersicon hirsutum, repels spider-mites. J Chem Ecol 19:2981–2997 Vavilov NI (1927) Geographical regularities in the distribution of the genes of cultivated plants. Bull Appl Bot Genet Plant Breed 17:411–428 Vidavsky F, Czosnek H (1998) Tomato breeding lines resistant and tolerant to tomato yellow leaf curl virus issued from Lycopersicon hirsutum. Phytopathology 88:910–914 Weigend M (2002) Observations on the biogeography of the Amotape-Huancabamba Zone in northern Peru. Bot Rev 68:38–54 Weir BS, Cockerham CC (1984) Estimating F-statistics for the analysis of population structure. Evolution 38:1358–1370 Yeh FC, Yang RC, Boyle TBJ, Ye ZH, Mao JX (1997) POPGENE, the user-friendly shareware for population genetic analysis. Molecular Biology and Biotechnology Centre, University of Alberta, Alberta. http://www.ualberta.ca/~fyeh/index.htm Young KR, Reynel C (1997) Huancabamba region, Perú and Ecuador. In: Davis SD, Heywood VH, Herrera-MacBryde O, Villa-Lobos J, Hamilton AC (eds) Centres of plant diversity: a guide and strategy for their conservation, 3: North America, Middle America, South America, Caribbean Island. IUCN, Cambridge Zuriaga E, Blanca J, Nuez F (2009a) Classification and phylogenetic relationships in Solanum section Lycopersicon based on AFLP and two nuclear gene sequences. Genet Resour Crop Evol 56:663–678 Zuriaga E, Blanca J, Cordero L, Sifres A, Blas-Cerdan W, Morales R (2009b) Genetic and bioclimatic variation in Solanum pimpinellifolium. Genet Resour Crop Evol 56:39–51

Report a bug

Under construction

We're in Beta!

The GoTriple platform is still in Beta and we keep adding new features everyday. Check the project's website to see what's new and subscribe to our Mailing List.