[EN] In vitro culture encompasses a set of techniques that allow the multiplication of plant material, vegetal material sanitation and genetic improvement of plants. These processes include techniques such as varieties of high commercial value, meristem culture, micropropagation of varieties of high commercial value, growing from meristems, the micrografts, genetic variability increased; both intraspecific using somaclonal variation and extraespecific by embryo rescue or somatic hybridization by fusion of protoplasts, obtaining haploid and doble-haploides plants and genetic transformation. The tomato is the most important horticultural species around the world after the potato. It´s use has spread practically by everyone, both fresh and in form of sauces, juices, concentrates, etc. There are several wild species phylogenetically related that may serve as sources of genetic variation improvement of the cultured species programs. In our laboratory we have done various methods of regeneration through induction of adventitious organogenesis of some wild species related to the tomato. It is the application in techniques like genetic transformation that has allowed us to obtain a very valuable material for the identification of key genes in multiple processes through a programme of insertional mutagenesis. In this context, the improvement of a method of regeneration or their implementation, in the case of work with a new species, can open new avenues in the investigation that our group is carrying out. Taking into account all of the above, the central objective of this project is to deepen the knowledge of the induction of adventitious organogenesis in Solanum lycopersicum var. Cerasiforme, an ancestor of the cultivated tomato and in Solanum pimpinellifolium and Solanum galapagense, two wild species of the same genus. In order to do so, we have studied the influence of different factors such as explant type, culture medium and incubation conditions on the induction of adventitious organogenesis. The development of these methods is of great importance for their application in improvement programs based on the induction of variability (i.e., somaclonal variation) or genetic transformation. TFGM