[EN] The variability of 62 Spanish landraces (and two hybrids used as reference) was described by analysing 39 morphological traits and eight SSR makers. Results showed that 81% of the examined genetic pool belonged to the inodorus type. Spanish traditional melons presented fruits from flattened and globular shapes to elliptical. Rind colour varied from pale green, almost white and yellow to dark green and almost black. Rind texture varied from smooth to intensely wrinkled. Spanish landraces also had larger fruits (average fruit weight 2.6 kg) and longer vegetative cycles (117 days to maturity) compared to landraces from other geographical origins. Farmers seem to have focused on selection towards large fruits, which usually requires the longest production cycles. Fruit colour, size and shape seemed to have been determinant in varietal selection. Hierarchical clustering resulted in two main groups (climacteric and non-climacteric). The largest group was composed of 60 accessions of non-climacteric types, which includes the most demanded by national markets, 'Piel de Sapo', which fruits were characterized by an ovate or elliptical shape, a green rind, big spots and stains distributed over the whole fruit, a rounded blossom end shape and a very pointed stem end shape. The study demonstrates that the Spanish genetic pool is much more diverse. Wide variability was found in a geographical area with vast historical importance in melon farming. These evaluations has allowed the identification of several uniform groups of non-climacteric cultivars ('Piel de Sapo', 'Mochuelo', 'Tendral', Yellow/White, Winter and Black groups) and a set of highly variable climacteric ones. However, many accessions with singular properties remain unclassified, demonstrating the morphological variability of the studied collection. Melons in Spain have wide variability together with a vast historical importance on farms. Some fruit types, or at least some morphological characters reflected by painters during centuries, have reached the present. The present study confirmed the need to preserve these irreplaceable genetic resources and continue their study and evaluation for valuable traits which could enhance farmer's opportunities for entering new markets. The authors would like to thank Maria Jose Diez, the curator of the COMAV's Genebank for providing some of the melon varieties included in this study. Financial support was received from the Genome-Spain Foundation through the Project MELONOMICS (http://www.gen-es.org/es/melanomics.cfm). This study was partially supported by the Project AGL2014-53398-C2-R (funded by the Spanish Economy and Competitiveness Department, and the European Regional Development Fund). Lázaro, A.; Fernandez, I.; Borrero, M.; Cabello, F.; Lopez-Sese, A.; Gómez-Guillamon, M.; Picó Sirvent, MB. (2017). Agromorphological genetic diversity of Spanish traditional melons. Genetic Resources and Crop Evolution. 64(7):1687-1706. doi:10.1007/s10722-016-0466-0 Camacho-Villa TC, Maxted N, Scholtena M, Ford-Lloyd B (2005) Defining and identifying crop landraces. Plant Genet Resour Charact Util 3(3):373–384 ECPGR Working Group on Cucurbits (2008) Minimum descriptors for Cucurbita spp., cucumber, melon and watermelon. ECPGR Secretariat, Rome, Italy Escribano S, Lázaro A (2009) Agro-morphological diversity of Spanish traditional melons (Cucumis melo L.) of the Madrid provenance. Genet Resour Crop Evol 56(4):481–497 Escribano S, Lázaro A (2012) Sensorial characteristics of Spanish traditional melon genotypes: has the flavor of melon changed in the last century? Eur Food Res Technol 234(4):581–592. doi: 10.1007/s00217-012-1661-7 Escribano S, Lázaro A, Cuevas HE, Lopez-Sese AI, Staub JE (2012) Spanish melons (Cucumis melo L.) of the Madrid provenance: a unique germplasm reservoir. Genet Resour Crop Evol 59(3):359–373 Esquinas-Alcázar JT, Gulik PJ (1983) Genetic resources of Cucurbitaceae: a global report. International Board of Plant Genetic Resources, Rome Esteras C, Nuez F, Picó MB (2011) Genetic diversity studies in cucurbits using molecular tools. In: Wang Y-H, Behera TK, Kole C (eds) Genetics, genomics and breeding of crop plants. Genetics, genomics and breeding of cucurbits. Science, pp 225–255 Esteras C, Formisano G, Roig C, Diaz A, Blanca J, Garcia-Mas J, Gomez-Guillamon ML, Lopez-Sese AI, Lazaro A, Monforte AJ, Pico B (2013) SNP genotyping in melons: genetic variation, population structure, and linkage disequilibrium. Theor Appl Genet 126(5):1285–1303 FAO (2013) FAOSTAT-trade and markets. http://www.fao.org/es/esc/en/index.html . Accessed 31 Mar 2016 Fernandez-Trujillo JP, Fernandez-Talavera M, Ruiz-Leon M, Roca MJ, Dos-Santos N (2012) Aroma volatiles during whole melon ripening in a climacteric near-isogenic line and its inbred non-climacteric parents. International Society of Horticulture Science, Leuven, p 1 Gao P, Ma H, Luan F, Song H (2012) DNA fingerprinting of Chinese melon provides evidentiary support of seed quality appraisal. PLoS ONE 7(12): e52431. doi: 10.1371/journal.pone.0052431 Hammer K, Gladis T (2014) Notes on infraspecific nomenclature and classifications of cultivated plants in Compositae, Cruciferae, Cucurbitaceae, Gramineae (with a remark on Triticum dicoccon Schrank) and Leguminosae. Genet Resour Crop Evol 61(8):1455–1467 Kerje T, Grum M (2000) The origin of melon, Cucumis melo: a review of the literature. Acta Hortic 510:37–44 Kimura M, Crow JF (1964) The number of alleles that can be maintained in a finite population. Genetics 49:725–738 Lázaro A, Fernández-Navarro I, de Lorenzo C (2016) Using landraces in agriculture, food and cooking: experiences around a big city in Southern Europe. In: Maxted N, Dulloo MF-LB (eds) Enhancing crop genepool use. CABI, Birmingham, pp 313–317 Levene H (1949) On a matching problem arising in genetics. Ann Math Stat 20:91–94 Lewontin RC (1972) The apportionment of human diversity. Evol Biol 6:381–398 López-Sesé AI, Staub JE, Gómez-Guillamón ML (2003) Genetic analysis of Spanish melon (Cucumis melo L.) germplasm using a standardized molecular-marker array and geographically diverse reference accessions. Theor Appl Genet 108(1):41–52 McCreight JD, Nerson H, Grumet R (1993) Melon, Cucumis melo L. In: Kalloo G, Berkg BO (eds) Genetic improvement of vegetable crops. Pergamon Press, New York, pp 267–294 Negri V, Becker H, Onnela J, Sartori A, Strajeru S, Laliberté B (2000) A first inventory of on-farm conservation and management activities in Europe including examples of formal and informal sector cooperation. In: Laliberté B, Maggioni L, Maxted N, Negri V (eds) ECP/GR In situ and on-farm conservation network. Report of a task force on wild species conservation in genetic reserves and a task force on on-farm conservation and management. Isola Polvese, Italy, pp 15–31 Nei M (1973) Analysis of gene diversity in subdivided populations. Proc Natl Acad Sci USA 70(12):3321–3323 Paris HS, Amar Z, Lev E (2012) Medieval emergence of sweet melons, Cucumis melo (Cucurbitaceae). Ann Bot 110(1):23–33 Pitrat M (2008) Melon (Cucumis melo L.). In: Prohens J, Nuez F (eds) Handbook of crop breeding: vegetables, vol 1. Springer, New York, pp 283–315 Roy A, Bal SS, Fergany M, Kaur S, Singh H, Malik A, Singh J, Monforte AJ, Dhillon NPS (2012) Wild melon diversity in India (Punjab State). Genet Resour Crop Evol 59(5):755–767 Sensoy S, Buyukalaca S, Abak K (2007) Evaluation of genetic diversity in Turkish melons (Cucumis melo L.) based on phenotypic characters and RAPD markers. Genet Resour Crop Evol 54(6):1351–1365 Spataro G, Negri V (2013) The European seed legislation on conservation varieties: focus, implementation, present and future impact on landrace on farm conservation. Genet Resour Crop Evol 60(8):2421–2430 Staub JE, Danin-Poleg Y, Fazio G, Horejsi T, Reis N, Katzir N (2000) Comparative analysis of cultivated melon groups (Cucumis melo L.) using random amplified polymorphic DNA and simple sequence repeat markers. Euphytica 115(3):225–241 Staub J, López-Sesé A, Fanourakis N (2004) Diversity among melon landraces (Cucumis melo L.) from Greece and their genetic relationships with other melon germplasm of diverse origins. Euphytica 136(2):151–166 Szamosi C, Solmaz I, Sari N, Barsony C (2010) Morphological evaluation and comparison of Hungarian and Turkish melon (Cucumis melo L.) germplasm. Sci Hortic 124(2):170–182 Tomason Y, Nimmakayala P, Levi A, Reddy U (2013) Map-based molecular diversity, linkage disequilibrium and association mapping of fruit traits in melon. Mol Breed 31(4):829–841 Tzitzikas EN, Monforte AJ, Fatihi A, Kypriotakis Z, Iacovides TA, Ioannides IM, Kalaitzis P (2009) Genetic diversity and population structure of traditional Greek and Cypriot melon cultigens (Cucumis melo L.) based on simple sequence repeat variability. HortScience 44(7):1820–1824

Lotti C, Marcotrigiano A, De Giovanni C, Resta P, Ricciardi A, Zonno V, Fanizza G, Ricciardi L (2008) Univariate and multivariate analysis performed on bio-agronomical traits of Cucumis melo L. germplasm. Genet Resour Crop Evol 55(4):511–522

Alvarez de Sotomayor JM (1824) Columela (AD 4–AD 70) Los Doce Libros de Agricultura. Traducidos al castellano por D.Juan María Alvarez de Sotomayor y Rubio, Santander Blanca J, Esteras C, Ziarsolo P, Perez D, Fernandez-Pedrosa V, Collado C, Rodriguez de Pablos R, Ballester A, Roig C, Canizares J, Pico B (2012) Transcriptome sequencing for SNP discovery across Cucumis melo. BMC Genomics 13:280. doi: 10.1186/1471-2164-13-280 Cubero JI (2001) El libro de agricultura de Al Awam/Edición y comentario sobre la traducción de Banqueri. Empresa Pública para el Desarrollo Agrario y Pesquero de Andalucía, Sevilla Herrera GA (1777) Agricultura general. Don Antonio de Sancha, Madrid Laguna A (1991) Pedacio Dioscorides anazarbeo, Acerca de la materia medicinal y de los venenos mortiferos. Consejería de Agricultura y Cooperación de la Comunidad, Secretaría General Técnica, Madrid Lázaro A, Fernández-Navarro I, Cabello F, de Lorenzo C (2010) Catálogo de variedadres de melón tradicionales españolas. IMIDRA, Madrid, ISBN-10: 84-695-6262-2. www.madrid.org/bvirtual/BVCM003438.pdf Rodriguez-Mohedano R, Rodriguez-Mohedano P (1781) Historia Literaria de España. Joachin Ibarra, Madrid

Alvarez de Sotomayor JM (1824) Columela (AD 4 — AD 70) Los Doce Libros de Agricultura. Traducdos al castellano por D.Juan María Alvarez de Sotomayor y Rubio, Santander Blanca J, Esteras C, Ziarsolo P, Perez D, Fernandez-Pedrosa V, Collado C, Rodriguez de Pablos R, Ballester A, Roig C, Canizares J, Pico B (2012) Transcriptome sequencing for SNP discovery across Cucumis melo. BMC Genomics 13: 280. doi: 10.1186/1471-2164-13-280 Cubero JI (2001) El libro de Agricultura de Al Awam/Edición y Comentario sobre la Traducción de Banqueri. Empresa Pública para el Desarrollo Agrario y Pesquero de Andalucía, Sevilla Herrera GA (1777) Agricultura general. Don Antonio de Sancha, Madrid Laguna A (1991) Pedacio Dioscorides anazarbeo, Aearch de la Material medicinal y de los venenos mortiferos. Consejería de Agricultura y Cooperación de la Comunidad, Secretaría General Técnica, Madrid Lázaro A, Fernández-Navarro I, Cabello F, de Lorenzo C (2010) Catálogo de variedadres de Melón tradicionales españolas. Imidra, Madrid, ISBN-10: 84-695-6262-2. www.madrid.org/bvirtual/BVCM003438.pdf Rodriguez-Mohedano R, Rodriguez-Mohedano P (1781) Historia Literaria de España. Joachin Ibarra, Madrid

Agromorphological genetic diversity of Spanish traditional melons

Abstract