The growing pressure on society due to global change requires better integration of ecosystem services (ES) into decision-making. Despite a growing number of ES assessments, Europe-wide information on recent changes of multiple ES is still rare. This study aimed at analysing changes in ES values between 2000 and 2018 across Europe based on land use/land cover (LULC) distribution. We mapped 19 ES for 52 ecoregions and identified six major groups of ecoregions with similar LULC distribution and trends. Our results indicated that provisioning ES mainly increased in the forest-dominated region (G2), decreasing in the near-natural grassland region (G1), the region with agricultural mixed systems (G3), and the intensively-used steppic region (G6). Regulating ES slightly decreased in G1 and G6, but increased in G2 and the wetland-dominated region (G5). Cultural ES had generally low negative trends for most ecoregions. In addition, our results revealed ecoregions with differing trends in ES that could be related to specific socioeconomic developments. Our findings provide spatial and quantitative information that can be used for policy development at European national and regional levels—as well as for monitoring of ES.