The hepatoprotective effect of the food supplementation with probiotic (Bacillus subtillis) and betaglucana in bull frogs subjected to the condition of tenderness has been assessed. The animals were distributed in the treatments: T1: 100 animals m-2 (control), T2: 236 animals m-2, T3: 236 m-2 animals supplemented with probiotic; and T4: 236 m-2 animals supplemented with betaglucana. Liver survival, weight gain (GP), biochemi-mica and histopathological condition has been assessed. No statistical difference for survival (P > 0,05) was observed, but significant differences were observed for GP at 30 days between unsupplemented and supplemented treatments (P < 0,001). Biochemi-mych assessments showed very close conditions between the animals in the control treatment and those supplemented with betaglucana. Histopathological analysis showed lesions and cytoplasmic rareage in 100 % of samples suggesting protein deficiency. Betaglucana has shown a hepatoprotective effect, reducing the effects of stagnation on bull frogs’ liver, but probiotic Bacillus subtillis did not have the same potential.