Culture influences the way people can be managed successfully according to the organizational objectives including sustainability. Hofstede´s cultural dimensions have been widely studied in different contexts. Dorfman and Howell designed an instrument in English to measure those dimensions. However, there is no validated Spanish version for this instrument. The objective of this article is to provide to the Ibero-American community a Spanish version of the Dorfman and Howell instrument. The questionnaire was translated into Spanish and adapted to the Chilean and Colombian populations. The study included 1136 participants, 500 from Chile and 636 from Colombia. Exploratory and confirmatory factor analysis were supported by the KMO and the Bartlett tests. Results indicate a better fit of a five-factor model, in similarity with the English language original instrument, as follows: masculinity–femininity, paternalism, power distance, uncertainty avoidance, and individualism–collectivism. Construct validity of the scales was confirmed in the Chilean sample, showing results consistent with previous meta-analytical research. The Dorfman and Howell instrument is a valid questionnaire for the evaluation of cultural dimensions in Spanish-speaking populations. The measurement of culture is a tool that leaders have available to facilitate the understanding and management of people. Organizations with operations in different countries or with intercultural context may use the results of this instrument to tune up their interventions.