Polianthes tuberosa L. is an endemic plant in Mexico, commercially grown for cut flowers, and used in the pharmaceutical and perfume industry. Traditionally, producers spread it using corms, which has meant that the crop has little genetic variability and for this reason only cultivars with white flowers are known. On the basis of the above, the objective of this investigation was to establish the practical and competitive methodology for propagating in vitro Polianthes tuberosa L. The yolks and small segments of corm tissue were placed on the surface of the growing medium, GC base (personal communication by Guillermo Carrillo) with the inorganic salts of the Murashige and Skoog culture medium (1962) per litre, 50 mL of coconut water, 20 g of sucrose, 6.4 g of agar and the pH was adjusted to 5.7. With this basic medium, culture media containing benzylaminopurine (BAP), naphthalenacetic acid (ANA), 2,4-dichlorophenoxyacetic acid (2,4-D), indollacetic acid (AIA) and cinetine were prepared. The formation of sprouts that gave rise to seedlings occurred through direct regeneration of floral yolk which, as it grows, forms only one seedling and from the tissue of the yolk base, up to six yolk shoots were obtained from this region in the medium containing BAP, 4.5 mg and ANA, 0.1 mg, of which the regeneration of sprouts was highest (56.1 %). In the case of cormo the problem was contamination, in some cases reaching up to 100 % of the crops. It is important to highlight the importance of being able to achieve mass in vitro regeneration of narcotic plants from floral buds.