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Article

Spanish

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oai:doaj.org/article:40fad990ca4847d5817d1126671cb9b1

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Efficiency of the temporary immersion system versus conventional in vitro propagation method

Abstract

The work was carried out with the aim of assessing the efficiency of the methods of mass propagation of vitroplants using results obtained in the conventional method and the temporary immersion system (SIT) and achieving increases in the multiplication coefficient, as well as characterising both methods of in vitro micropropagation by describing them. Whereas conventional methods of propagation would be slow to produce and have high production costs. This technique makes it possible to draw up appropriate planning from the stage of establishment of the aseptic crop in the laboratory to the establishment and production of the crop in the field. However, in temporary immersion crops, the constitution and physiological process of the sprouts are very similar to those shown in ex vitro environments due to the evolution of the environment of the system. Several authors, including Cruzat, (2009), states that the use of ITS reduces production costs per plant; as a result of the mechanisation of some micropropagation stages and Winkelman et al (2006), I comment as in bioreactors, obtaining seedlings allows the production costs to be reduced by 5060 %. Results corroborating the efficiency of the temporary immersion system against the conventional in vitro propagation method. Keywords: Temporary immersion, vitroplants, efficiency. ABSTRACT The work was carried out with the aim of assessing the efficiency of the methods of mass propagation of vitroplants through results obtained in the Conventional Method and the Temporary immersion System (SIT) and achieved increases in the multiplication efficient, in addition to characterising methods of in vitro micropropagation by Describing them. Considering that conventional propagation methods would be low to produce and of high production costs. This technique Applicant for proper planning, from the stage of establishment of Aseptic culture in the laboratory, to the establishment and production of the crop in the field. However, in temporary immersion crops, the constitution and Physiological process of outcomes are very similar to others in ex vitro environments, due to the evolution of the system environment. Severe authors, including Cruzat, (2009), states that the use of the SIT reduces production costs per plant; this as a result of the mechanisation of some stages of micropropagation and Winkelman, et al, (2006), commenting as in bioactors, obtaining Seedlings appliances the reduction of production costs by between 50 and 60 %. Results that corroborate the efficiency of the temporary immersion system against the conventional in vitro propagation method. Keywords: Temporary immersion, vitroplants, efficiency.

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