Growing public concern about the negative effects of agricultural activities on the environment has led to more sustainable crop production, especially in the management of fertilisation. Composta is an organic fertiliser used as a source of nutrients and as a component of substrates in protected agriculture. The objective of this study was to assess the effect of different substrates based on compost of cattle (C) mixed with river sand (A) on the yield and biophysical and nutraceutical quality of tomato fruits produced under greenhouse conditions. The percentages of the substrates were: 25: 75, 50: 50, 75: 25 (C: (A) and as a control the Steiner nutrient solution was used in sand. The variables assessed were yield, average fruit weight, polar and equatorial diameter, pericarp thickness and total soluble solids (biophysical quality). Nutraceutical quality was determined by analysis of lycopene and antioxidant activity. The highest biophysical yield and quality of the fruit came with the use of the conventional nutrient solution. On the other hand, fruit obtained from compost: sand 75: 25 gave the highest values for soluble solids and antioxidant capacity, 12.5 % higher for soluble solids and 27 % higher in antioxidant capacity compared to those obtained with Steiner nutrient solution. Composta combination with sand for use as organic substrate is a viable alternative for the organic production of tomato in greenhouses as yield and quality are acceptable. Furthermore; given the potential for use in certified organic farming, users will eventually be able to achieve an overcharge and improve the benefit-cost ratio, which provides competitive marketing potential for the producer, while reducing the use of inorganic inputs, thereby contributing to the preservation of the environment.