Working people spend around 54% of their waking hours at a workplace, according to recent statistics. Work-related stress is unavoidable, and it can damage the health of employees and affect business performance. In this paper, we argue that open space inside the workplace environment can have a positive influence on reducing overall stress levels in all the categories of users. To our knowledge, there is a significant lack of research considering specific business districts and the gated complexes called business parks, especially in post-socialist Eastern European cities, where there they are still a novelty. Empirical research in this study is on the single case study of Business Park “Airport city” in Belgrade, Serbia. Its main focus is on the survey conducted with 235 participants based on a questionnaire, which examines the relation between workplace stress and workplace environments. The findings from the questionnaire show that the frequency, duration, and activity of open space usage influence the stress levels of employees in this specific workplace, while it is not visible relating to their age and gender. Additionally, final implications suggest that improved open space, such as well-expected greenery, but also the urban design non-associative to workspace and the socialization and exercise amenities customized for frequent and short work breaks, can facilitate the overall well-being of employees. They are innovative elements in relatively underdeveloped research on stress measures with open space usage characteristics in the specific (gated) workplace setting.