Tobacco is mainly used to produce cigarettes and cigars, which are consumed worldwide. However, this habit is not beneficial for users and the World Health Organisation is seeking to reduce this use for tobacco. To this end, it is necessary for other uses of the tobacco plant not only for the smoking sector. One of the alternatives to the use of tobacco cultivation is to extract the oil from its seeds for the production of biodiesel and other bio-products. To this end, a study was carried out to optimise extraction in the ERT 60 screw press (Scott Tech) by comparing two extra gloves as follows: the pattern and the optimised one, and two types of tobacco seed: residual Virginia and Solaris. It was noted that using optimised gloves increased the extraction yield by 8.72 % for residual Virginia seed and by 11.49 % for Solaris seed in relation to standard extra glove. It was concluded that the established objective of optimising oil extraction by pressing from tobacco seed has been achieved.