Initially, timber was considered only as an easily accessible and processable material in nature; however, its excellent properties have since become better understood. During the discovery of new building materials and thanks to new technological development processes, industrial processing technologies and gradually drastically decreasing forest areas, wood has become an increasingly neglected material. Load-bearing structures are made mostly of reinforced concrete or steel elements. However, ecological changes, the obvious problems associated with environmental pollution and climate change, are drawing increasing attention to the importance of environmental awareness. These factors are attracting increased attention to wood as a building material. The increased demand for timber as a building material offers the possibility of improving its mechanical and physical properties, and so new wood-based composite materials or new joints of timber structures are being developed to ensure a better load capacity and stiffness of the structure. Therefore, this article deals with the improvement of the frame connection of the timber frame column and a diaphragm beam using mechanical fasteners. In common practice, bolts or a combination of bolts and pins are used for this type of connection. The subject of the research and its motivation was to replace these commonly used fasteners with more modern ones to shorten and simplify the assembly time and to improve the load capacity and rigidity of this type of frame connection.