The article provides data on the use of a complex preparation, which includes sodium selenite and tocopherol (vitamin E) in the form of injections for sterlet breeders (Acipenser ruthenus Linnaeus, 1758). Obtained data confirm the ability of E-selenium to regulate metabolic processes towards the formation of reproductive tissue by optimizing them. Positive dynamics is manifested during the gametogenesis, in particular, the number of individuals with reproductive cells at the IV stage of maturity is increased.