Introduction: The Asparagaceae family includes several genders adapted to survive at high temperatures and with low water availability. These include Agave, Beaucarnea, Dasylirion, Nolina and Yucca. Mexico has a high biodiversity of these plant groups, as well as an ancestral tradition in their use. In vitro propagation systems are efficient for the production of these plants and can also be used for various types of studies. These include heavy metal tolerance studies, which can be used to determine which species are suitable for reforestation and regeneration of areas contaminated by mining activities. Method: In this work, 20 species of the Asparagaceae family were spread in vitro and their rooting process in culture media added with Cd, Cu, Cr, Fe, Mn, Pb and Zn was evaluated. Root development in the presence of these metals was analysed and their capacity to absorb them was determined by atomic flame absorption spectrophotometry. With this data, translocation and bioaccumulation factors were calculated in order to characterise the response of each species to heavy metals. Results: The highest number of outbreaks occurred in Beaucarnea recurvata with 5.7 and Nolina durangensis with 5.6 outbreaks per explorer, respectively. Treatment with chromium was toxic to most species. Agave celsii, A. chiapensis, A. obscura, A. palmeri and A. salmiana formed roots in all metal treatments. According to the translocation and bioaccumulation factors, the species analysed were classified as phytostabilisers, excluders, bioaccumulators and indicators. The species A. chiapensis, A. obscura and A. palmeri were bioaccumulators for Mn. A. celsii and A. salmiana presented phytostabilisation and exclusion mechanisms. Conclusion: In vitro spread makes it possible to generate large quantities of plants and also to study their responses to the presence of contaminants. The genus Agave proved to be metal tolerant and can therefore be used to plant stabilise and remediate contaminated soils.